Target background:
Serotonin or 5-hydroxytryptamine is a monoamine neurotransmitter. It’s synthesized in serotonergic neurons in the central nervous system and enterochromaffin cells in the gastrointestinal tract. Because these neurons profile diffusely throughout the brain, serotonin can affect various brain functions. It also interacts with many other neurotransmitters, either directly through neurons that use both serotonin and another neurotransmitter, or by serotonin neurons influencing neurons that primarily use these other transmitters.
Target alias:
5 hydroxytryptamine, 5HT, 25689, 5 HT5A, 5 HT, 5HT 15563, 5HT15563, 5 HT 5A
Specificity: Formaldehyde conjugate of serotonin. Without paraformaldehyde treatment, it cross-reacts in liquid phase with dopamine and tryptamine in haemagglutination tests. The antibody reacts with serotonin in neurons and recognises serotonergic sites in fixed tissue sections. It does not react with catecholamine-containing neurons in formaldehyde fixed preparations. By immunocytochemistry no cross-reactivity with dopamine and noradrenaline (6). By red cell agglutination, no cross-reactivity with 5-hydroxyindolacetic acid, GABA, noradrenaline, 5-hydroxytryptophan, carnosine, melatonin
Recommend starting dilution:
If reconstituted with deionized water in 0.5 mL: IHC 1:100 - 1:200 for formaldehyde fixed tissue. Optimal dilution has to be determined by the user.
References:
1.-Consolazione A - Immunocytochemical detection of serotonin with monoclonal antibodies.
Storage:
Lyophilized antibodies can be kept at 4ºC for up to 3 months and should be kept at -20ºC for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80ºC) or kept at 4ºC for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy.
