Target:
Pseudomonas Aeruginosa antigen O5
Target background:
Pseudomonas aeruginosa is a Gram-negative bacterium widely distributed in nature and causing opportunistic infections in humans. P. aeruginosa is an important bacterial pathogen of nosocomial (hospital derived) infections, and it can also cause life threatening diseases in patients with cancer, burn wounds, cystic fibrosis and those that have received immunosuppressive therapy. Classification of P. aeruginosa isolates is an important and routine task in hospitals. The basis for the serotyping system for P. aeruginosa is the differences among the O antigen side chains of lipopolysaccharide (LPS). A large number of O antigen side chains have been established by various investigators around the world which led to the creation of a standardized serotyping system known as the International Antigenic Typing Scheme (IATS) with twenty characterized O serotype strains of P. aeruginosa.
Target alias:
P. Aeruginosa antigen O5, serotype 5
Recommend starting dilution:
Reconstitute with deionized water. Optimal dilution has to be determined by the user.
References:
1.-Lam JS - LPS quantitation procedures.
2.-Kaluzny K - Coexistence of two distinct versions of O-antigen polymerase, Wzy-alpha and Wzy-beta, in Pseudomonas aeruginosa serogroup O2 and their contribution...
3.-Daniels C - Pseudomonas aeruginosa O-antigen chain length is determined before ligation to lipid A core.
4.-Newton GJ - Three-component-mediated serotype conversion in Pseudomonas aeruginosa by bacteriophage D3.
5.-Rahim R - Involvement of the rml locus in core oligosaccharide and O polysaccharide assembly in Pseudomonas aeruginosa.
6.-Dasgupta T - Characterization of lipopolysaccharide-deficient mutants of Pseudomonas aeruginosa derived from serotypes O3, O5, and O6.
7.-Lightfoot J and Lam JS - Chromosomal mapping, expression and synthesis of lipopolysaccharide in Pseudomonas aeruginosa: a role for guanosine diphospho (GDP)-D-mannose.
8.-Lam JS - Monoclonal antibodies as probes to examine serotype-specific and cross-reactive epitopes of lipopolysaccharides from serotypes O2, O5, and O16 of P...
9.-Lam MY - Occurrence of a common lipopolysaccharide antigen in standard and clinical strains of Pseudomonas aeruginosa.
10.-Lam JS - Visualization of Pseudomonas aeruginosa O antigens by using a protein A-dextran-colloidal gold conjugate with both immunoglobulin G and immunoglobu...
11.-Lam JS - Production and characterization of monoclonal antibodies against serotype strains of Pseudomonas aeruginosa.
Storage:
Lyophilized antibodies can be kept at 4ºC for up to 3 months and should be kept at -20ºC for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80ºC) or kept at 4ºC for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy.